St James' holds treasures dating back to the earliest days of the colony of Melbourne, and some of these can be seen in our museum, situated in the room behind the pulpit at the front of the church. These include a painting and bust of the first Dean of Melbourne, Hussey Burgh Macartney; the original plans for the church by Robert Russell; and a handwritten collection of sermons by Reverend Grylls, who pastored the colony while St James' was being built.

We are presently engaged in a process of cataloguing and re-archiving the various records and artefacts in our possession to ensure they can be enjoyed by future generations. At times this will mean the museum will be closed to the public. Please contact the church office in advance to confirm accessibility. The museum can be opened for visitors on request during the church's office hours.